Each year there are approximately 8.9 million cases of osteoporosis which mostly occurs in women aged over 50 years.
Use of synthetic drugs that are often used to reduce the process of resorption and prevent osteoporosis there are many causes side effects.
This is the background for five students of Faculty of Pharmacy to look for other alternatives to the use of natural ingredients safer, effective, and efficient, ie utilizing the oyster mushrooms.
Yana Bintoro Priambodo, Rien Larasati Arini, Aida Fathia, Devyanto Hadi, and Mega Asri daughter was doing research that aims to understand the process of osteoclastogenesis inhibitory effect of oyster mushroom extracts in vitro and in silico.
"In the case of osteoporosis, an imbalance of osteoblast and osteoclast be the main drivers of this disease. If the formation of osteoclasts (osteoclastogenesis) can be inhibited, then the process of bone resorption is reduced," said Yana, Tuesday (2/8).
Therefore, inhibition of osteoclastogenesis can be used as a strategic approach for the treatment of osteoporosis.
Osteoblasts and osteoclasts, he said, is a component that plays a role in the process of osteoporosis or bone mass reduction caused by an imbalance between bone resorption and bone formation. As a result, patients often feel pain, lose the ability to move, and more at risk of broken bones (fractures).
Oyster mushrooms have been selected as the research object because it is known to contain lovastatin potential as an alternative inhibiting osteoclastogenesis process. Because the compound is able to increase osteoblast differentiation and inhibit osteoclastogenesis.
On the other hand, the oyster mushroom is also one of confectionary which is currently quite popular consumption and have a lot of nutrients such as protein, fiber, carbohydrates, and low in fat. In this study, they used modeling RAW264 macrophage cells induced by osteoclast differentiation factor (ODF).
The study was conducted by observing the activity of osteoclastogenesis by the method of Tartrate-Resistant Acid Phosphatase (TRAP) staining as well as to predict the interaction between lovastatin binding to RANK-RANKL and MAP kinase by using molecular docking.
From research conducted, they found, the data in vitro oyster mushrooms can become anti-osteoporosis agent and reduce the risk of osteoporosis by inhibiting osteoclastogenesis process.
"This study is the first step to discover the oyster mushrooms for an anti-osteo that prospective, cheap, safe. The results of this study are expected to provide an effective and specific innovations in the treatment of osteoporosis targeted through inhibition of osteoclastogenesis," said Yana.